品牌:美國 NewEast Biosciences
貨號(hào): 80103
規(guī)格:96 well
產(chǎn)品全名:非乙?；痗GMP ELISA試劑盒
標(biāo)記:His6
Product Highlight
Based on the unique mouse monoclonal anti-cGMP antibody
The only assay kit on the market with monoclonal anti-cGMP antibody (all other kits are based on polyclonal anti-cGMP antibodies)
The monoclonal anti-cGMP antibody displays much higher selectivity over cAMP and GTP, than polyclonal anti-cGMP antibody
Avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility
Product Description
Guanosine 3′, 5′-cyclic monophosphate (cyclic GMP; cGMP) plays critical regulatory roles in many physiological processes. cGMP is produced from GTP by guanylyl cyclases and is degraded by phosphodiesterases. Stimulation of guanylyl cyclases or inhibition of phosphodiesterases can increase cellular cGMP concentrations. Inhibitors of the cGMP-specific phosphodiesterases are used for treating human diseases. For example, inhibitors of cGMP specific phosphodiesterase type 5 (such as Viagra and Cialis) are used for treating erectile dysfunction.
To screen for inhibitors of phosphodiesterases or stimulators of guanylyl cyclases, it is essential to have a sensitive, selective and reproducible method to measure the cGMP concentrations. This is especially true for the initial screenings given the possible weaker effects of larger pools of compounds.
Currently available other ELISA kits measuring cGMP levels are based on the non-affinity-purified polyclonal anti-cGMP antibody. Despite the claimed selectivity, these polyclonal anti-cGMP antibodies display certain cross-reactivity with cAMP or GTP. In most cell types, cGMP is present at levels ~100 fold lower than cAMP.
NewEast Biosciences cGMP ELISA kit is based on the unique mouse monoclonal anti-cGMP antibody. This monoclonal anti-cGMP antibody displays >108fold of selectivity over cAMP, GTP and other nucleoside analogues. NewEast Biosciences cGMP ELISA kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cGMP antibodies. Our monoclonal anti-cGMP antibody-based ELISA kit also avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility in the long run.
Principle Outline
NewEast Biosciences cGMP ELISA Kit is a competitive immunoassay to measure the cGMP levels, either from cell extracts or from in vitro guanylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cGMP in cell extracts or in in vitro guanylyl cyclase assays will competitively bind to the monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cGMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cGMP in samples. The measured optical density is used to calculate the concentration of cGMP in samples based on the curve from the cGMP standards.
武漢費(fèi)斯德生物科技有限公司是美國NewEast Biosciences在中國的辦事處，也是目前所有產(chǎn)品國內(nèi)經(jīng)銷處。
目前客戶使用產(chǎn)品發(fā)表超過1000谷歌學(xué)術(shù)文獻(xiàn)，其中論文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研發(fā)倆種獨(dú)特的抗體。這倆種抗體僅僅識(shí)別活性的GTP酶或者突變的Oncogene。 GTP酶涉及(1)響應(yīng)細(xì)胞表面受體**的信號(hào)轉(zhuǎn)導(dǎo)，包括跨膜受體，例如介導(dǎo)味覺、嗅覺和視覺的那些，（2）核糖體的蛋白質(zhì)生物合成，（3）調(diào)節(jié)細(xì)胞分化、增殖、分裂和運(yùn)動(dòng)，（4）蛋白質(zhì)通過膜的易位，（5）細(xì)胞內(nèi)囊泡的運(yùn)輸，以及囊泡介導(dǎo)的分泌和攝取，通過GTP酶控制囊泡外殼組裝。Oncogene側(cè)是誘發(fā)癌癥的基因。
更多產(chǎn)品類目和技術(shù)資料可查看：武漢費(fèi)斯德生物官網(wǎng)。
