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Chenomx NMR Suite結(jié)合了復(fù)雜的化學(xué)和高等算法,可通過廣泛的代謝物光譜模型庫識別和量化NMR光譜中的代謝物。
Chenomx NMR分析軟件可與綜合光譜參考庫一起使用,在一個集成的工作流程中識別和測量NMR光譜中可見化合物的濃度。該軟件提供濃度測量、識別和光譜去卷積。
Chenomx NMR分析軟件光譜參考庫是根據(jù)通過NMR分析獲得的數(shù)千個參考光譜開發(fā)的,用于識別和比較目的。參考庫的pH值范圍為4到9,NMR場強(qiáng)范圍為400MHz到800MHz。
Chenomx NMR分析軟件通過將光譜參考庫“擬合”到實驗光譜中的適當(dāng)信號來發(fā)揮作用。該軟件會自動調(diào)整參考庫以反映樣品和采集條件(pH、NMR場強(qiáng)、譜線寬度),并允許用于調(diào)整峰強(qiáng)度和位置以獲得微調(diào)的濃度值。
擬合實驗信號后,頻譜的殘差線就暴露出來了。分析軟件將比較光譜參考庫中的更多化合物,以分離和識別剩余的信號。
以下是Chenomx NMR Suite的核心模塊:
Processor
Processor模塊可以讓您將各種NMR光譜導(dǎo)入Chenomx NMR Suite。它還提供清理和校準(zhǔn)這些光譜所需的標(biāo)準(zhǔn)處理工具,以便您可以使用Profiler模塊更輕松、更準(zhǔn)確地分析它們。
Profiler
Profiler模塊可讓您通過將混合光譜與具有數(shù)百個參考特征的化合物庫進(jìn)行比較來識別和量化混合光譜的內(nèi)容。
Library Manager
Library Manager模塊讓您管理可用于Chenomx NMR Suite中全部其他模塊的化合物集。這包括您使用Compound Builder創(chuàng)建的自定義化合物,以及全部標(biāo)準(zhǔn)Chenomx參考化合物。
Spin Simulator
Spin Simulator模塊允許您基于一些理論細(xì)節(jié)來模擬整個復(fù)合簽名。只需設(shè)置一個磁體頻率,然后開始求解耦合關(guān)系并測量 j 值常數(shù),同時您的仿真會實時自我更新!
限Professional版
Compound Builder
Compound Builder模塊允許您創(chuàng)建自己的自定義化合物簽名,并包含定量信息。然后可以在Profiler中分析樣本時使用這些簽名。
Batch Functions
批量處理顯著減少了大樣本量的處理時間。批量導(dǎo)入/批量處理將處理步驟應(yīng)用于樣本組;而Batch Fit/Batch Edit將來自單個光譜的分析化合物應(yīng)用于整批光譜,以減少分析時間。然后,添加星星、別針和顏色進(jìn)行標(biāo)記。
Spectral Binning
Spectral Binning模塊通過將光譜劃分為一系列ppm區(qū)域或bin來減少變量的數(shù)量;隨后的分析涉及這些箱的綜合區(qū)域,而不是原始光譜數(shù)據(jù)。
COMPLETE Autofit
全新的自動迭代檔案器,可執(zhí)行多種并行方法來自動擬合NMR光譜,并生成多個“BEST”擬合的CNX文件。
Chenomx NMR Suite目前支持以下格式的原始數(shù)據(jù):
Agilent (fid)
Bruker (fid)
JEOL (.jdf)
NMRPipe (.fid)
并以這些格式處理數(shù)據(jù):
Agilent (phasefile)
Bruker (1r)
JEOL (.jdf)
NMRPipe (.ft2)
JCAMP-DX (.jdx, version 5.1 and higher)
Mestrelab MNOVA
Chenomx NMR Suite的第10版引入了COMPLETE Autofit:一種能夠?qū)θ幚淼墓庾V進(jìn)行全自動分析的新工具。
COMPLETE Autofit是一款全新的自動迭代檔案器,它可以執(zhí)行多種并行方法來完成NMR光譜的自動擬合,然后確定低的統(tǒng)計誤差,并提供“BEST”擬合。
V10的新增功能:
重新引入舊版Autofit(8.6及更早版本)
添加富馬酸鹽和馬來酸鹽作為內(nèi)標(biāo)。
改進(jìn)的復(fù)合峰簇自動定位
可重復(fù)使用的基線斷點位置
以二進(jìn)制和TXT格式批量導(dǎo)出光譜數(shù)據(jù)
以JCAMP格式批量導(dǎo)出
應(yīng)用領(lǐng)域
1. 細(xì)胞培養(yǎng)
代謝物分析已被證明是觀察和了解培養(yǎng)細(xì)胞代謝表型的寶貴工具。在大型生物反應(yīng)器條件下,存在很多挑戰(zhàn),例如優(yōu)化生長培養(yǎng)基、生長補(bǔ)料策略和其他工程參數(shù),這些參數(shù)不只對細(xì)胞生長而且對您的產(chǎn)品生產(chǎn)很關(guān)鍵。借助Chenomx的Profiling技術(shù),獲得可靠的定量數(shù)據(jù)是克服這些挑戰(zhàn)的關(guān)鍵。
2. 食物和營養(yǎng)
從必需氨基酸到各種糖類和其他緊要的食物成分(如抗氧化劑),定量測量食物的營養(yǎng)成分是了解食品之間差異的關(guān)鍵。了解與飲食相關(guān)的疾病或生長因素,以及它們與食物攝入的相關(guān)性對于我們爭取更好的健康很關(guān)鍵。早期的營養(yǎng)干預(yù)可能會預(yù)防未來可能需要藥物幫助的疾病的發(fā)作。
3. 藥物代謝
隨著藥物開發(fā)成本的飆升,充分了解您的藥物靶點變得如此關(guān)鍵。代謝組學(xué)可以成為藥物發(fā)現(xiàn)的關(guān)鍵工具。從早期毒性檢測到細(xì)胞水平的作用機(jī)制,識別和量化相關(guān)生物流體中的代謝物可以彌合對化合物的理解差距。從藥物開發(fā)管道的臨床前到臨床階段,額外的定量代謝物數(shù)據(jù)將為您的藥物提交增加真正的價值。Chenomx在血清、血漿、尿液、腦脊液、唾液、組織和基于細(xì)胞的模型方面擁多年經(jīng)驗。
4. 生物標(biāo)志物發(fā)現(xiàn)
發(fā)現(xiàn)新的生物標(biāo)志物一直是臨床研究人員了解疾病機(jī)制并幫助治療這些疾病的目標(biāo)。NMR不只提供了一種涵蓋各種分子類型的分析方法,而且與 Chenomx技術(shù)相結(jié)合,隨著對樣品中成分的鑒定和量化的發(fā)現(xiàn),該技術(shù)變得更加有價值。Chenomx擁有多年設(shè)計實驗、構(gòu)建診斷模型以及使用臨床試驗數(shù)據(jù)驗證它們的經(jīng)驗。
系統(tǒng)要求
Chenomx NMR suite可用于:
Windows 10 / 8 / 7 / Vista(64 位)
Mac OS X 10.14 – 10.10(64 位)
Linux x86(64 位)
Chenomx NMR Suite只適用于64位操作系統(tǒng),因為Java不再支持32位體系結(jié)構(gòu)。
【英文介紹】
Chenomx NMR Suite combines sophisticated chemistry and advanced algorithms to identify and quantify metabolites in NMR spectra with an extensive library of spectral models of metabolites.
Used by Metabolomics researchers world-wide, Chenomx NMR Analysis Software works with comprehensive Spectral Reference Libraries to both identify and measure concentrations of compounds visible in the NMR spectra, all in one integrated workflow. This patented software offers best-in-class concentration measurement, identification and advanced de-convolution of spectra.
The Chenomx NMR Analysis SoftwareSpectral Reference Libraries are developed from thousands of reference spectra obtained through NMR analysis and used for identification and comparison purposes. The Reference Libraries are available from pH 4 through 9 and NMR field strengths from 400 MHz through 800 MHz.
Chenomx NMR Analysis Software functions by 'fitting' the Spectral Reference Library to the appropriate signals within the experimental spectrum. The software automatically adjusts the Reference Library to reflect the sample and acquisition conditions (pH, NMR field strength, spectra line width) and allows the used to adjust peak intensity and location for fine-tuned concentration values.
After the experimental signal has been fit, the spectrum's residual line is exposed. The profiling software will compare more compounds from the Spectral Reference Library to isolate and identify the remaining signals.
Core Modules
Processor
The 'Processor' module lets you import a wide variety of NMR spectra into Chenomx NMR Suite. It also provides the standard processing tools needed to clean up and calibrate those spectra, so that you can more easily and accurately analyze them using the Profiler module.
Profiler
The 'Profiler' module lets you identify and quantify the contents of your mixture spectrum by comparing it to a compound library with hundreds of reference signatures.
Library Manager
The 'Library Manager' module lets you manage sets of compounds that are available to all other modules in Chenomx NMR Suite. This includes any custom compounds that you've created with Compound Builder, as well as all of the standard Chenomx Reference Compounds.
Spin Simulator
The 'Spin Simulator' module allows you to simulate an entire compound signature based on a few theoretical details. Just set a magnet frequency, then start solving coupling relationships and measuring j-value constants while your simulation updates itself in real time!
Professional Version Only
Compound Builder
The 'Compound Builder' module allows you to create your own custom compound signatures, complete with full quantitative information. These signatures can then be used when analyzing samples in Profiler.
Batch Functions
Batching significantly reduces processing time for large sample sizes. Batch Import/Batch Process applies processing steps to groups of samples; while Batch Fit/Batch Edit applies profiled compounds from a single spectra to an entire batch of spectra to reduce profiling time. Then, add stars, pins, and colors for labelling.
Spectral Binning
Binning reduces the number of variables by dividing spectra into a series of ppm regions, or bins; subsequent analysis involves the integrated area of these bins instead of the raw spectral data.
COMPLETE Autofit
All-new Automatic Iterative Profiler that performs numerous, parallel approaches to a complete autofit of your NMR spectra and generates multiple CNX files with a recommended‘BEST’ fit.