3D全自動外泌體熒光檢測分析系統(tǒng)
法國Abbelight公司開發(fā)的3D全自動外泌體熒光檢測分析系統(tǒng)是一款無需純化的、全自動的可對單個外泌體進行表征分析的全新設備。該設備能夠提供外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達、單個外泌體的膜蛋白與生物標志物共定位等。操作簡單，結果可靠。
3D全自動外泌體熒光檢測分析系統(tǒng)基于特異性免疫捕獲技術，允許研究者直接分析特定群體的外泌體。通過單分子定位技術成像，可以得到單個外泌體的超分辨成像結果，尺度可以到20nm。全自動外泌體熒光檢測分析系統(tǒng)兼容各種生物樣本，除了純化的外泌體之外，對于血液、尿液、惡性腫瘤、腹水中的外泌體也可直接檢測分析，大大拓展了研究范圍。
應用方向及主要特征
無須純化 | |
| 特異性捕獲 | |
| 超分辨成像與粒徑統(tǒng)計 | |
| 表面蛋白與內容物分析 | |
| 多通道成像 | |
| 單外泌體共定位分析 |
超高分辨單外泌體成像
左：2D單個外泌體成像；右：3D單個外泌體成像
外泌體粒徑分析：通過團簇分析外泌體的整體和亞群的粒徑分布
膜蛋白表征與共定位分析，以CD63，CD81&CD9為例表征其單陽，雙陽和三陽的比例
外泌體內容物表征
對比穿膜處理后外泌體中內容物aGFP的表達
觀測細胞中外泌體的分布情況，研究細胞中細胞器或者其他蛋白和外泌體的定位關系
應用案例
法國Abbelight公司開發(fā)的3D全自動外泌體熒光檢測分析系統(tǒng)是一款無需純化的、全自動的可對單個外泌體進行表征分析的全新設備。該設備能夠提供單個外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達、單個外泌體的膜蛋白與生物標志物共定位等。
應用領域：腫瘤診斷，藥系統(tǒng)開發(fā)，眼科疾病診斷，疫苗研發(fā)，脊髓受傷機制研究，血漿/血清外泌體分析，外泌體工程化，呼吸疾病診斷
測試數據：
外泌體2D成像
外泌體3D成像
外泌體形態(tài)分析
發(fā)表文章
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用戶單位
