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產(chǎn)品熱度：加載中

品　　牌：ROWIAK

產(chǎn)品型號(hào)：TissueSurgeon

適用范圍：高教

所在地區(qū)：德國(guó)

雙光子3D組織切割成像系統(tǒng)-TissueSurgeon

　　——OCT圖像引導(dǎo)的組織和材料的非接觸精確切割，更適合切小鼠脛骨等小鼠骨骼系統(tǒng)，牙齒的激光切片設(shè)備

　　應(yīng)用領(lǐng)域

　　■  骨科方面，尤其是非脫鈣硬組織和種植體界面研究

　　■  心臟病學(xué)和心血管研究與醫(yī)學(xué)，尤其是軟組織與生物材料和支架，鈣化斑塊研究

　　■  再生醫(yī)學(xué)與組織工程學(xué)，尤其是植入物、支架等研究

　　■  口腔、面部和牙科醫(yī)學(xué)，尤其是非脫鈣硬組織，金屬、陶瓷或聚合物植入物研究

　　■  耳鼻喉相關(guān)研究，如耳蝸、耳蝸植入物等

　　■  從小鼠到大型動(dòng)物模型的臨床前研究等

　　為何選用TissueSurgeon？

　　■  樣本損失小：幾乎連續(xù)切片非脫鈣硬組織，無需大深度磨片，材料損失小；

　　■  難切割樣本：硬組織、軟組織、軟硬結(jié)合組織切割，甚至脆弱的樣本(如耳蝸)切割；

　　■  適合界面研究：種植體組織界面組織學(xué)（如牙釘、心血管支架、支架）；

　　■  無接觸切割：無接觸激光切割組織可避免擠壓、劃傷或裂紋等；

　　■  用3D切片方法可以沿著牙釘種植體-組織界面對(duì)特定部位的樣本進(jìn)行定向、溫和的分離；

　　■  切割過程不會(huì)污染、灼傷或機(jī)械力損傷樣品，可用于生物化學(xué)分析的無污染和無接觸樣品的制備；

　　■  用于組織工程的生物材料切割（如支架、聚四氟乙烯、水凝膠）；

　　■  組織，基質(zhì)和材料的3D微結(jié)構(gòu)切割；

　　■  薄切片厚度：硬組織切片10 μm；

　　■  切片速度：≥1 mm2/s；

　　■  光源類型：紅外飛秒脈沖激光；

　　■  光學(xué)相干斷層掃描（OCT）引導(dǎo)切割，可以測(cè)量樣品尺寸和層厚，并能夠定位到病變部位，直接對(duì)病變部位進(jìn)行切割，大大提高了切割效率。

　　設(shè)備參數(shù)

　　TissueSurgeon產(chǎn)品升級(jí)！激光組織學(xué)的新維度：超大尺寸和可調(diào)節(jié)

可以實(shí)現(xiàn)最大6.6 cm樣本切割

　　應(yīng)用案例

　　■  TissueSurgeon可視化切片系統(tǒng)，實(shí)現(xiàn)邊看邊切

　　對(duì)于病理等多種研究來書，涉及到組織切片的內(nèi)容， 困難的部分莫過于尋找病變部位。 相比一個(gè)完整組織來說， 有時(shí)候研究者所關(guān)注的部分僅僅是其中變異的一小部分組織的形態(tài)而已。 但是對(duì)于傳統(tǒng)切片手段來說， 缺乏一種有效的手段來定位這個(gè)區(qū)域， 因此往往需要投入大量人力和物力去多次制樣，大量切片來尋找這個(gè)部位。 TissueSurgeon 自身集成了適合深層組織細(xì)胞成像的光學(xué)相干斷層掃描（OCT）成像功能， 幫助您直接定位到 ROI 區(qū)域。 讓切片變得可視化， 實(shí)現(xiàn)更加和可控的切片。為研究者更加迅速直觀的找到病變位置，大大提高了研究效率。

對(duì)含有金屬釘?shù)墓趋肋M(jìn)行成OCT成像，并引導(dǎo)切片

　　■  原位細(xì)胞3D切割成像技術(shù)基于青鳉胚胎組織的單細(xì)胞提取

　　單細(xì)胞的原位組織提取一直以來都是一項(xiàng)十分困難的工作，這主要受制于組織之間連接致密難以消化，而機(jī)械力往往很難地將單個(gè)細(xì)胞與組織完整的分離。激光切割具有傳統(tǒng)切割技術(shù)所難以匹及的切割精度，是目前一種比較理想的切割手段，因此圍繞激光切割技術(shù)的相關(guān)顯微產(chǎn)品也孕育而生，并在科研領(lǐng)域中越來越受到關(guān)注。但是激光切割也有其局限性，先顯微激光切割往往要從表面開始，無法對(duì)深層組織進(jìn)行切割；另一方面激光的光源往往采用紫外激光光源，這種類型的光源很容易造成組織灼傷，從而影響切割下來樣品的品質(zhì)，因此激光切割的應(yīng)用發(fā)展也受到了諸多限制。

　　如今ROWIAK公司推出的一款全新的單細(xì)胞分離系統(tǒng)有望解決這一難題。它采用了近紅外雙光子激光切割技術(shù)，在保留了激光切割精度優(yōu)勢(shì)的同時(shí)，采用近紅外波長(zhǎng)的激光從而避免了激光切中對(duì)組織灼燒的問題。因此能夠?qū)崿F(xiàn)的原位組織中的單個(gè)細(xì)胞的分離。

　　青鳉是一種成熟的模式生物，常用于分析發(fā)育和發(fā)育過程中的細(xì)胞信號(hào)神經(jīng)生物學(xué)研究。其中使用表達(dá)熒光蛋白的轉(zhuǎn)基因胚胎是一種揭示胚胎發(fā)育的良好方法。隨著基因技術(shù)的發(fā)展，研究者們?cè)絹碓蕉嗟亻_始關(guān)注這些標(biāo)記細(xì)胞中轉(zhuǎn)錄組中的信息。雖然單細(xì)胞測(cè)序技術(shù)發(fā)展迅速，但是從組織中獲得單細(xì)胞的手段卻十分有限。目前幾乎沒有手段能夠直接在組織的原位上快速獲取一個(gè)細(xì)胞，但是基于ROWIAK雙光子切割技術(shù)，研究者成功地在這方面取得了一些進(jìn)展。

青鳉胚胎中感知神經(jīng)中表達(dá)mcherry的細(xì)胞成像

　　研究者為了研究青鳉感覺神經(jīng)分泌細(xì)胞細(xì)胞群中特定表達(dá)m-cherry的轉(zhuǎn)基因細(xì)胞的內(nèi)部遺傳信息，將ROWIAK雙光子3D組織切割成像系統(tǒng)與傳統(tǒng)的顯微操作系統(tǒng)進(jìn)行結(jié)合，成功實(shí)現(xiàn)了對(duì)目標(biāo)細(xì)胞的原位分離。

　　研究者先利用雙光子3D組織切割成像系統(tǒng)對(duì)青鳉胚胎中的mcherry細(xì)胞進(jìn)行了定位，然后根據(jù)其細(xì)胞群的形態(tài)設(shè)定了切割部位，隨后系統(tǒng)根據(jù)預(yù)先設(shè)定的范圍進(jìn)行切割。待切割完成后使用玻璃微管移液器將目標(biāo)的細(xì)胞部位直接取出，即獲得了目標(biāo)組織區(qū)域。這種方法能夠在不破壞樣品原位信息的情況下將感興趣的部位直接的分離，這對(duì)于揭示生物體的基因表達(dá)情況具有著深遠(yuǎn)的意義。

從青鳉胚胎中分離特定表達(dá)mcherry的細(xì)胞團(tuán)

　　參考文獻(xiàn)：

　　Wittbrodt, J. et al. Medaka — a model organism from the Far East. Nature Reviews Genetics 3, 53-64.

　　Yamamoto, T. (ed.) MEDAKA (Killifish): Biology and strains. Yamamoto, T. (ed.) Keigaku Pub. Co., Tokyo, 1975, pp.365.

　　Kristin Tessmar-Raible et al.Removal of fluorescently-labeled sensory-neurosecretory cells from forebrain of transgenic Medaka embryos, focusonmicroscop. 2011.

　　測(cè)試數(shù)據(jù)

　　發(fā)表文章

　　1. Nolte, P.; Brettmacher M.; Gr?ger, C. J.; Gellhaus, T.; Svetlove A.; Schilling, A. F.; Alves, A.; Ru?mann, C.; Dullin, C.; (2023) Spatial correlation of 2D hardtissue histology with 3D microCT scans through 3D printed phantoms Sci Rep 13, 18479 

　　2.  Kevin Janot, Grégoire Boulouis, Géraud Forestier, Fouzi Bala, Jonathan Cortese, Zoltán Szatmáry, Sylvia M. Bardet, Maxime Baudouin, Marie-Laure Perrin, Jérémy Mounier, Claude Couquet, Catherine Yardin, Guillaume Segonds, Nicolas Dubois, Alexandra Martinez, Pierre-Louis Lesage, Yong-Hong Ding, Ramanathan Kadirvel , Daying Dai, Charbel Mounayer, Faraj Terro, Aymeric Rouchaud. (2023) WEB shape modifications: “angiography–histopathology correlations in rabbits” J NeuroIntervent Surg 2023;0:1–7. 

　　3. Géraud FORESTIER, Jonathan CORTESE, Sylvia M. BARDET, Maxime BAUDOUIN, Kévin JANOT, Voahirana RATSIMBAZAFY, Marie-Laure PERRIN, Jérémy MOUNIER, Claude COUQUET, Catherine YARDIN, Yan LARRAGNEGUY, Flavie SOUHAUT, Romain CHAUVET, Alexis BELGACEM, Sonia BRISCHOUX, Julien MAGNE, Charbel MOUNAYER, Faraj TERRO, Aymeric ROUCHAUD. (2023) “Comparison of Arterial Wall Integration of different Flow Diverters in rabbits” the CICAFLOW study Journal of Neuroradiology, In press. 

　　4. Donath, S?ren, Leon Angerstein, Lara Gentemann, Dominik Müller, Anna E. Seidler, Christian Jesinghaus, André Bleich, Alexander Heisterkamp, Manuela Buettner, and Stefan Kalies. (2022). “Investigation of Colonic Regeneration via Precise Damage Application Using Femtosecond Laser-Based Nanosurgery” Cells 11, no. 7: 1143. https://doi.org/10.3390/cells11071143 

　　5. Müller, Dominik, S?ren Donath, Emanuel G. Brückner, Santoshi Biswanath Devadas, Fiene Daniel, Lara Gentemann, Robert Zweigerdt, Alexander Heisterkamp, and Stefan M.K. Kalies. (2021). “How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes” Bioengineering 8, no. 12: 213. https://doi.org/10.3390/bioengineering8120213 

　　6. Müller D, Klamt T, Gentemann L, Heisterkamp A, Kalies SMK (2021) Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes. PLoS ONE 16(6): e0252346. https://doi.org/10.1371/journal.pone.0252346

　　7. Bouyer M; Garot C; Machillot P; Vollaire J; Fitzpatrick V; Morand S; Boutonnat J; Josserand V; Bettega G; Picart C (2021) 3D-printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect Materials Today Bio 11 100113 

　　8. Verhaegen C, Kautbally S, Zapareto D C, Brusa D, Courtoy G, Aydin S, Bouzin C, Oury C, Bertrand L, Jacques P J, Beauloye C, Horman S, Kefer J (2020) Early thrombogenicity of coronary stents: comparison of bioresorbable polymer sirolimus-eluting and bare metal stents in an aortic rat model. Am J Cardiovasc Dis. 10(2):72-83 

　　9. Zeller-Plumhoff B, Malicha C, Krüger D, Campbella G, Wiesea B, Galli S, Wennerberg A, Willumeit-R?mer R, Wieland F (2020) Analysis of the bone ultrastructure around biodegradable Mg–x Gd implants using small angle X-ray scattering and X-ray diffraction Acta Biomaterialia 101 637–645

　　10. Rousselle S D , Wicks J R, Tabb B C, Tellez A, O’Brien M (2019) Histology Strategies for Medical Implants and Interventional Device Studies Toxicologic Pathology Vol. 47(3) 235-249 

　　11. Neuerburg C, Mittlmeier L M, Keppler A M, Westphal I, Glass ?, Saller M M, Herlyn P K E, Richter H, B?cker W, Schieker M, Aszodi A, Fischer D C (2019) Growth factor-mediated augmentation of long bones: evaluation of a BMP-7 loaded thermoresponsive hydrogel in a murine femoral intramedullary injection model. Journal of Orthopaedic Surgery and Research 14 297 

　　12. Kunert-Keil C, Richter H, Zeidler-Rentzsch I, Bleeker I, Gredes T (2019) Histological comparison between laser microtome sections and ground specimens of implant-containing tissues. Annals of Anatomy 222 153–157 

　　13. Gabler C, Sa? JO, Gierschner S, Lindner T, Bader R, Tischer T (2018) In Vivo Evaluation of Different Collagen Scaffolds in an Achilles Tendon Defect Model. BioMed Research International 208

　　14.    Wolkers W, Vásquez-Rivera A, Oldenhof H, Dipresa D, Goecke T, Kouvaka  A, Will F, Haverich A, Korossis S, Hilfiker A (2018) Use of sucrose to diminish pore formation in freeze-dried heart valves. Scientific Reports 8 12982 

　　15. Albers J, Markus MA, Alves F, Dullin C (2018) X-ray based virtual histology allows guided sectioning of heavy ion stained murine lungs for histological analysis. Scientific Reports 8(1) 7712

　　16. Boyde A (2018) Evaluation of laser ablation microtomy for correlative microscopy of hard tissues. Journal of Microscopy 271(8) 1-14

　　17.    Pobloth AM, Checa S, Razi H, Petersen A, Weaver JC, Schmidt-Bleek K, Windolf M, Tatai Aá, Roth CP, Schaser KD, Duda GN, Schwabe P (2018) Mechanobiologically optimized 3D titanium-mesh scaffolds enhance bone regeneration in critical segmental defects in sheep. Science Translational Medicine 10 423

　　18. Joner M, Nicol P, Rai H, Richter H, Foin N, Ng J, Cuesta J, Rivero F, Serrano R, Alfonso F (2018) Very Late Scaffold Thrombosis: Insights from Optical Coherence Tomography and Histopathology. EuroIntervention 13(18)

　　19. Boyde A, Staines KA, Javaheri B, Millan JL, Pitsillides AA, Farquharson C (2017) A distinctive patchy osteomalacia characterises Phospho1 deficient mice. Journal of Anatomy 231 298-308

　　20. Kowtharapu BS, Marfurt C, Hovakimyan M, Will F, Richter H, Wree A, Stachs O, Guthoff RF (2017) Femtosecond laser cutting of human corneas for the subbasal nerve plexus evaluation. Journal of Microscopy 265(1) 21–26

　　21. Will F, Richter H (2015) Laser-based Preparation of Biological Tissue. Laser Technik Journal 12(5) 44-47

　　22. Richter H, Ratliff J, Will F, Stolze B (2015) Time- and material saving laser microtomy for hard tissue and implants. European Cells and Materials 29 Suppl.2 4

　　23. Richter H, Ramirez Ojeda DF, Will F (2014) Lasergesteuerte Probenpr?paration von Hartgeweben und Biomaterialien. BIOspektrum 05 14

　　24. Bourassa D, Gleber S-C, Vogt S, Yi H, Will F,  Richter H, Shin CH, Fahrni CJ (2014) 3D Imaging of Transition Metals in the Zebrafish Embryo by X-ray Fluorescence Microtomography. Metallomics 6 1648-1655

　　25. Schimek K, Busek M, Brincker S, Groth B, Hoffmann S, Lauster R, Lindner G, Lorenz A, Menzel U, Sonntag F, Walles H, Marx U, Horland R. (2013) Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip 13 3588-3598

　　26. Richter H, Ratliff J (2012) A Non-Contact Method of Sectioning Cardiovascular Arteries Containing Metallic Stents Using Laser Technology. J Histotechnol 35 (4) 205

　　27. Richter H, Lubatschowski H, Will F (2011) Laser in Medizin & Biologie: Laser-Mikrotomie mit ultrakurzen Pulsen – Neue Perspektiven für die Gewebe- und Biomaterialbearbeitung. Biophotonik 09 50-52

　　28. Lubatschowski H, Will F, Przemeck S, Richter H (2011) Laser Microtomy. Handbook of Biophotonics Vol. 2: Photonics for Health Care Wiley-VCH 151-157 

　　29. Kermani O, Will F, Massow O, Oberheide U, Lubatschowski H (2010) Control of Femtosecond Thin-flap LASIK Using OCT in Human Donor Eyes. Journal of Refractive Surgery 26(1) 57-61

　　30. Baumgart J, Bintig W, Ngezahayo A, Lubatschowski H, Heisterkamp A (2010) Fs-laser-induced Ca2+ concentration change during membrane perforation for cell transfection. Optics Express 18 (3) 2219

　　31. Kermani O, Will F, Massow O, Oberheide U, Lubatschowski H. (2009) Echtzeitsteuerung einer Femtosekundenlaser Sub-Bowman-Keratomileusis an humanen Spenderaugen mittels optischer Koh?renztomographie. Klin Monatsbl Augenheilkd 226 965-969

　　32. Kütemeyer K, Baumgart J, Lubatschowski L, Heisterkamp A (2009) Repetition rate dependency of low density plasma effects during femtosecond-laser-based surgery of biological tissue. Appl. Phys. B 97(3) 695

　　33. Baumgart J, Kuetemeyer K, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A (2009) Repetition rate dependency of reactive oxygen species formation during femtosecond laser-based cell surgery. J Biomed Opt 14(5) 054040

　　34. Kermani O, Will F, Lubatschowski H (2008) Real-Time Optical Coherence Tomography-Guided Femtosecond Laser Sub-Bowman Keratomileusis on Human Donor Eyes. Am J Ophthalmol 146 42–45.

　　35. Kermani O (2008) ?Sehendes Skalpell” schon heute realisierbar. Ophthalmologische Nachrichten 09 (Kongressausgabe)

　　36. Baumgart J, Bintig W, Ngezahayo A, Willenbrock S, Murua Escobar H, Ertmer W, Lubatschowski H, Heisterkamp A (2008) Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53a cells. Opt. Express 16(5) 3021-3031

　　37. Baumgart J, Kuetemeyer K, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A (2008) Investigation of reactive oxygen species in living cells during femtosecond laser based cell surgery. Proc. SPIE Optical Interactions with Tissue and Cells XIX Vol 6854

　　38. Heisterkamp A, Baumgart J, Maxwell IZ, Ngezahayo A, Mazur E, Lubatschowski H (2007) Fs-Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells, and Studies of Cell Mechanics. Laser Manipulation of Cells and Tissues; Elsevier Inc. 293-307

　　39. Will F, Block T, Menne P, Lubatschowski H (2007) Laser Microtome: all optical preparation of thin tissue samples. Proceedings of SPIE 6460 646007-1

　　40. Lubatschowski H (2007) Laser Microtomy – Opening a new Feasibility for Tissue Preparation. Optic & Photonic WILEY-VCH 49 – 51

　　41. Menne P (2007) Microtomy with Femtosecond Lasers. Biophotonics International; Laurin Publishing Co. Inc. May 2007 35 – 37

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