Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.
High binding capacity of 20 mg human IgG/mL.
High selectivity and low nonspecific adsorption.
Well suited for immunoprecipitation procedures.
Use in immunoprecipitation procedures
Protein A Sepharose CL-4B resin offers high specificity and capacity, making it dependable for precise, reproducible immunoprecipitation procedures – whether the end goal is determining protein quality, analyzing protein interactions, or assessing post-translational modifications.
| Parameter | Protein A Sepharose CL-4B, 1.5 g |
|---|---|
| Chromatography technique | Antibody affinity chromatography |
| Ligand | Native Protein A |
| Particle size, d50V1 | ~90 μm |
| Matrix | Cross-linked agarose, 4% |
| Particle Size | 45 μm-165 μm |
| Ligand Coupling Method | Cyanogen bromide activation |
| Binding Capacity/ml Chromatography Medium | ~20 mg human lgG/mL resin |
| Ligand density | 3 mg Protein A/ml medium |
| pH stability, operational2 | 3–9 |
| pH stability, CIP3 | 2–10 |
| Flow velocity | < 150 cm/h at 25°C, HR 16/10 columns, 5 cm bed height |
| Storage | 2 to 8°C, 20% Ethanol |
| Chemical stability | Stable in commonly used aqueous buffers, 6 M guanidine-HCl, 8 M urea |
| Sanitization Conditions | 2% Hibitane/20% Ethanol or with 70% Ethanol |
| Pack size | 1.5 g |
