| Description | An un-tagged recombinant protein corresponding to S. aureus Glutamyl endopeptidase Source:E. coli Amino Acid Sequence: MLPNNDRHQI TDTTNGHYAP VTYIQVEAPT GTFIASGVVV GKDTLLTNKH VVDATHGDPH ALKAFPSAIN QDNYPNGGFT AEQITKYSGE GDLAIVKFSP NEQNKHIGEV VKPATMSNNA ETQVNQNITV TGYPGDKPVA TMWESKGKIT YLKGEAMQYD LSTTGGNSGS PVFNEKNEVI GIHWGGVPNE FNGAVFINEN VRNFLKQNIE DIHFANDDQP NNPDNPDNPN NPDNPNNPDE PNNPDNPNNP DNPDNGDNNN SDNPDAA |
| Specificity | Glutamyl endopeptidase is a monomer, 28.9 kDa (267 aa) |
| Preparation Method | Protein is derived from genetically modified E. coli. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Purity | >95%, by SDS-PAGE |
| Endotoxin Note | Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method). |
Applications/Dilutions
| Theoretical MW | 28.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5 |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95%, by SDS-PAGE |
| Reconstitution Instructions | Sterile water at 0.1 mg/mL |
Notes
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Alternate Names for Recombinant S. aureus Glutamyl endopeptidase Protein
Endoproteinase Glu-C
Staphylococcal serine proteinase
V8 protease
