F(ab')2fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species.
Physical State:Sterile-filtered liquid
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.Expiration date:one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6Preservative:NoneSuggested Working Concentration or Dilution Range:
10-20 μg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
