

Features and advantages of QUANTI-Blue? Solution:
A convenient, easy and rapid means to detect and quantify SEAP and AP activity
Short hands-on-time: < 10 min
Easy to prepare: just mix QB Reagent and QB Buffer with water
Highly sensitive
Wide dynamic range
High saturation threshold
Allows for high-throughput screening (HTS)
In the presence of AP or SEAP, the color of QUANTI-Blue? changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
Figures
QUANTI-Blue? Standard procedure
QUANTI-Blue? High Throughput Screening (HTS) procedure
Specifications
Physicochemical characterization: pH and solubility
Functional characterization:cellular assays using alkaline phosphatase of SEAP-expressing reporter cells.
Contents
QUANTI-Blue? Solution is available in three pack sizes:
rep-qbs : 5 x 1 ml of QB reagent + 5 x 1 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 25 x 96-well plates or 20 x 1536-well plates
rep-qbs2 :10 x 1 ml of QB reagent + 10 x 1 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 50 x 96-well plates or 40 x 1536-well plates
rep-qbs3 :1 x 20 ml of QB reagent + 1 x 20 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 100 x 96-well plates or 80 x 1536-well plates
Store QB reagent and QB buffer at -20°C.
Reconstituted QUANTI-Blue? Solution is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue? away from light.