Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.
High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
Supplied as pre-swollen bulk (loose) resin.
Chemically stable with all commonly used aqueous buffers.
Suitable for batch GST tagged protein purification and scale-up.
Used in GST pull-down assays to study protein-protein interactions.
| Parameter | Glutathione Sepharose 4B, 10 mL |
|---|---|
| Chromatography technique | GST-tagged protein purification |
| Ligand | Glutathione |
| Particle size, d50V1 | ~90 μm |
| Matrix | Agarose, 4% |
| Particle Size | 45 μm-165 μm |
| Ligand Coupling Method | Epoxy activation |
| Binding Capacity/ml Chromatography Medium2 | > 25 mg horse liver GST/mL resin |
| pH stability, operational3 | 4–13 |
| pH stability, CIP4 | 4–13 |
| Storage | 4 to 30°C, 20% Ethanol |
| Chemical stability | Commonly used aqueous buffers |
| Licensing | For licensing information, see the Licensing Statements page in the About Us section. |
| Pack size | 10 mL |
