Zymoprep? Yeast Plasmid Miniprep II (50 Preps)
About Zymoprep? Yeast Plasmid Miniprep II
The Zymoprep? Yeast Plasmid Miniprep II provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Colitransformation, PCR, and Southern blot analysis.
| Format | Spin Column |
|---|---|
| Binding Capacity | ≤ 5 μg |
| Elution Volume | ≥ 10 μl |
| Plasmid DNA Size | Up to 23 kb (For plasmids >15 kb add elution water or TE and incubate for up to 5 minute before centrifugation). |
| Compatibility | S. cerevisiae, C. albicans, S. pombe, and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase?). |
Protpcol：Microsoft Word - Zymoprep-II-Protocol-9-9-04.doc產(chǎn)品官網(wǎng)鏈接：http://www.zymoresearch.com/dna/plasmid-dna-purification/yeast-plasmid/zymoprep-yeast-plasmid-miniprep-ii
