Alexa Fluor? 647 IgG Fraction Monoclonal Mouse Anti-Rabbit IgG
Target: RabbitHost:MouseAntibody Format:IgG Fraction MonoclonalSpecificity: IgG, light chain specific
Minimal Cross Reactivity:Bovine, Goat, Armenian Hamster, Horse, Human, Mouse, Rat, Sheep Immunoglobulins
Conjugate:Alexa Fluor? 647Product Category:Anti?Light Chain-Specific Antibodies for Western Blotting after IPClonality:MonoclonalRRID:AB_2339171
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rabbit IgG and with those common to other rabbit immunoglobulins. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, armenian hamster, horse, human, mouse, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State:Freeze-dried solid
Storage and Rehydration:Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration:Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.Expiration date:one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)Preservative:0.05% Sodium AzideSuggested Working Concentration or Dilution Range:
1:100 - 1:800 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Alexa Fluor? 647
Amax:651 Emax:667nm
Alexa Fluor? 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Alexa Fluor? 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. Alexa Fluor? 647 conjugates are the best choice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.
A significant advantage of using Alexa Fluor? 647 over lower wavelength-emitting dyes is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor? 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor? 647 is not recommended for use with conventional epifluorescent microscopes. It is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. Alexa Fluor? 647 conjugates are less expensive alternatives to allophycocyanin conjugates for flow cytometry.
