Product data and images for CD4 (L3T4) MicroBeads, mouse
Figures
Figure 1
A single-cell suspension from mouse spleen was prepared using the gentleMACS? Dissociator. CD4
+
T cells were isolated from this single-cell suspension using the CD4 (L3T4) MicroBeads, an LS Column, and a QuadroMACS? Separator. Cells were fluorescently stained with REAfinity? Antibodies CD45-VioGreen?, CD4-VioBlue
?
and CD8a-PE-Vio
?
770 and analyzed by flow cytometry using the MACSQuant
?
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
