| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
|---|---|
| Binding Capacity | 400 μg |
| Culture Input | ≤ 50 ml |
| Elution Volume | ≥ 100 μl |
| Endotoxin Levels | ≤ 0.025 EU/μg Plasmid DNA |
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge |
| Format | Spin Column |
| Processing Time | 20 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Size Range | Up to 200 kb |
| Yield | ≤ 400 μg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coliutilized) |
| at # | Name | Size |
|---|---|---|
| D4200 | ZymoPURE II Plasmid Midiprep Kit | 25 Preps. |
| D4201 | ZymoPURE II Plasmid Midiprep Kit | 50 Preps. |
