Physical State:Freeze-dried solid
Storage and Rehydration:Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration:Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.Expiration date:one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Alexa Fluor? 594 AffiniPure Fab Fragment Donkey Anti-Rabbit IgG (H+L)
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.
Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Purity:The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)Preservative:0.05% Sodium AzideSuggested Working Concentration or Dilution Range:
1:100 - 1:800 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
