MC1000 8通道藻類培養(yǎng)與在線監(jiān)測(cè)系統(tǒng)由8個(gè)100ml藻類培養(yǎng)試管、水浴控溫系統(tǒng)、LEDs光源控制系統(tǒng)及光密度和溶解氧(選配)在線監(jiān)測(cè)系統(tǒng)等組成,可用于藻類培養(yǎng)與控制實(shí)驗(yàn)、梯度對(duì)比實(shí)驗(yàn)等,適于水體生態(tài)毒理學(xué)研究檢測(cè)、藻類生理生態(tài)研究、水生態(tài)研究等,其主要功能特點(diǎn)如下:
1. 8通道藻類培養(yǎng),每個(gè)藻類培養(yǎng)試管可培養(yǎng)85ml藻液
2. LEDs光源,可對(duì)每個(gè)培養(yǎng)試管獨(dú)立調(diào)節(jié)控制和設(shè)置光強(qiáng)度和時(shí)間,如晝夜變化等
3. 光密度在線監(jiān)測(cè),包括OD680、OD735,監(jiān)測(cè)數(shù)據(jù)自動(dòng)存儲(chǔ)
4. 溶解氧在線監(jiān)測(cè)(備選)以測(cè)量分析藻類光合作用等
5. 溫度、光照控制可用戶設(shè)置不同的程序模式
6. 氣泡混勻:可通過(guò)調(diào)節(jié)閥手動(dòng)調(diào)節(jié)氣流量以對(duì)培養(yǎng)試管內(nèi)的藻類進(jìn)行混勻
7. 可選配O2/CO2監(jiān)測(cè)系統(tǒng),在線監(jiān)測(cè)藻類光合放氧和CO2吸收
8. 可選配藻類熒光測(cè)量模塊
應(yīng)用領(lǐng)域:
l 多通道同步藻類培養(yǎng)
l 同步梯度脅迫實(shí)驗(yàn)
l 培養(yǎng)條件優(yōu)化
l 控制培養(yǎng)條件與藻類生長(zhǎng)動(dòng)力學(xué)監(jiān)測(cè)
儀器型號(hào):
MC 1000:僅進(jìn)行藻類培養(yǎng),不能監(jiān)測(cè)OD
MC 1000-OD:可同時(shí)進(jìn)行藻類培養(yǎng)和OD監(jiān)測(cè)
技術(shù)指標(biāo):
1. 藻類同步培養(yǎng)通道:8個(gè)
2. 培養(yǎng)管容量:100ml,建議培養(yǎng)容量85ml
3. 在線即時(shí)監(jiān)測(cè)參數(shù):分別監(jiān)測(cè)每個(gè)培養(yǎng)管的OD680和OD720,數(shù)據(jù)自動(dòng)保存到主機(jī)內(nèi)存中,PIN光電二極管監(jiān)測(cè)器,665-750nm帶通濾波器(MC 1000-OD)
4. OD測(cè)量程序:將主機(jī)內(nèi)存中的OD數(shù)據(jù)下載到電腦中并以圖表形式顯示,數(shù)據(jù)可導(dǎo)出為T(mén)XT或Excel文件
5. 精確控溫范圍:標(biāo)準(zhǔn)配置20℃ - 60℃,可選配置15℃-60℃(需加配制冷單元)
6. 加熱系統(tǒng):150W筒形加熱器
7. 水浴體積:5L
8. 水浴自動(dòng)補(bǔ)水模塊(選配):水浴水位因蒸發(fā)降低后可自動(dòng)補(bǔ)水
9. 光源系統(tǒng):光強(qiáng)0-可調(diào),冷白光LED(標(biāo)配),強(qiáng)度可達(dá)900μmol/m2/s;暖白光LED(可選),光強(qiáng)可達(dá)750μmol/m2/s;其他顏色LED可定制
10. 控光模式:可靜態(tài)或動(dòng)態(tài)設(shè)置光照程序,如正弦、晝夜節(jié)律、脈沖等;可選配用戶自定義程序,支持用戶編輯多達(dá)224步的不同光強(qiáng)和持續(xù)時(shí)間的光周期
11. 控制單元顯示屏:可調(diào)控培養(yǎng)程序和顯示數(shù)據(jù)
12. 氣流調(diào)控:通過(guò)多管調(diào)節(jié)閥對(duì)8個(gè)培養(yǎng)管手動(dòng)獨(dú)立調(diào)控氣體流量
13. PBR實(shí)時(shí)在線監(jiān)測(cè)分析軟件(選配):
a) 通過(guò)PBR軟件動(dòng)態(tài)調(diào)控光照和溫度模式
b) 通過(guò)光密度(OD680、OD720)變化實(shí)時(shí)監(jiān)測(cè)藻類生物量
c) 對(duì)生長(zhǎng)速率進(jìn)行實(shí)時(shí)回歸分析
d) 多數(shù)據(jù)管理功能(過(guò)濾、查找、多重導(dǎo)出)
e) 可將測(cè)量數(shù)據(jù)、培養(yǎng)程序和其他信息保存到數(shù)據(jù)庫(kù)中
f) 通過(guò)GUI圖形用戶界面設(shè)置培養(yǎng)程序并在線顯示測(cè)量數(shù)據(jù)圖
g) 數(shù)據(jù)可導(dǎo)出為CSV、Excel或XML文件
14. GMS高精度氣體混合系統(tǒng)(選配):可控制氣體流速和成分,標(biāo)配為控制氮?dú)?空氣和二氧化碳,氣源需用戶自備
15. O2/CO2監(jiān)測(cè)系統(tǒng)(選配):8通道續(xù)批式監(jiān)測(cè)藻類CO2吸收或光合放氧通量:
a) 氧氣分析測(cè)量:氧氣測(cè)量范圍0-100%,分辨率0.0001%,精確度優(yōu)于0.1%,溫度、壓力補(bǔ)償,數(shù)碼過(guò)濾(噪音)0-50秒可調(diào),具兩行文字?jǐn)?shù)字LCD背光顯示屏,可同時(shí)顯示氧氣含量和氣壓
b) 二氧化碳分析測(cè)量:雙波長(zhǎng)非色散紅外技術(shù),測(cè)量范圍0-5%或0-15%兩級(jí)選擇(雙程),分辨率優(yōu)于0.0001%或1ppm(可達(dá)0.1ppm),精確度1%,通過(guò)軟件溫度補(bǔ)償,具兩行文字?jǐn)?shù)字LCD背光顯示屏,可同時(shí)顯示CO2含量和氣壓,具數(shù)碼過(guò)濾(噪音)功能
c) 氣體抽樣與氣路切換:具備隔膜泵、氣流控制針閥和精密流量計(jì),氣路自動(dòng)定時(shí)切換功能
16. 藻類熒光測(cè)量模塊(選配):用于測(cè)量藻類熒光參數(shù)以反映藻類生理狀態(tài)及濃度,熒光測(cè)量程序包括Ft,QY,OJIP-test,NPQ、光響應(yīng)曲線等,可選配探頭式測(cè)量或試管式測(cè)量:
a) 探頭式測(cè)量:具備光纖測(cè)量探頭,可插入培養(yǎng)液中原位測(cè)量藻類熒光參數(shù)
b) 試管式測(cè)量:具備測(cè)量杯,可取樣精確測(cè)量藻類熒光參數(shù)及光密度值
17. 通訊方式:RS232串口
18. 尺寸:71×33×21 cm
19. 重量:13kg
20. 供電:110-240V
應(yīng)用案例:
不同CO2濃度下衣藻Chlamydomonas的生長(zhǎng)曲線(Zhang,2014)
聚球藻Synechococcus野生型和△nblA的生長(zhǎng)曲線(Yu,2015)
產(chǎn)地:捷克
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