眾所周知，每個(gè)行業(yè)及產(chǎn)品線，都會(huì)在每年的時(shí)候產(chǎn)生銷量王，那么細(xì)胞因子/重組蛋白里面又有誰(shuí)成為今年的銷量王呢？跟隨小編一起了解一下。
在整個(gè)銷售排行榜單當(dāng)中，另外們驚訝的發(fā)現(xiàn)rHuApo-SAA1這樣一個(gè)重組蛋白的名字，這個(gè)產(chǎn)品對(duì)于小編來(lái)說(shuō)，好像只看懂了rHu代表著重組人，其他好像一概沒(méi)看明白。但是作為一個(gè)勤奮好學(xué)的小編，怎么可能就到此為止，翻閱資料檢索網(wǎng)絡(luò)就是基本的操作，那么一起來(lái)看看我的成果吧。
rHuApo-SAA1，全稱Recombinant Human Apolipoprotein-Serum Amyloid A1，翻譯出來(lái)應(yīng)該叫做重組人載脂蛋白血清淀粉樣蛋白A1，oh my god！中文名字就這么長(zhǎng)，我只能說(shuō)，哪些科學(xué)家們，真的厲害！請(qǐng)收下小編的膝蓋?。ㄎ艺娴奶y了）。
對(duì)于這個(gè)產(chǎn)品的描述是這樣的：
Serum amyloid A proteins(SAA)represents a family of apolipoproteins that circulates in association with high-density lipoproteins(HDL).The level of apo-SAA,normally 1-5μg/ml in plasma,increases 500-1000 fold within 24hoursof an inflammatory stimulus and,under these conditions,is the most abundant HDL apo-lipoprotein.The human SAA gene codes for a 122 amino acid non-glycosylated polypeptide,which contains an 18 amino acid N-terminal sequence.Recombinant human apo-SAA1 is an 11.7 kDa protein containing 104 amino acid residues.
為了方便大家查看，小編還是翻譯了一下，盡可能做到準(zhǔn)確，僅供大家參考：
血清淀粉樣蛋白a（saa）是一個(gè)載脂蛋白家族，與高密度脂蛋白（hdl）結(jié)合循環(huán)。在炎癥刺激的24小時(shí)內(nèi)，血漿中的apo-saa水平（通常為1-5μg/ml）增加500-1000倍，在這些條件下，是豐富的hdl-apo-脂蛋白。122個(gè)氨基酸非糖基化多肽，含18個(gè)氨基酸n末端序列，重組人apo-saa1為11.7kda蛋白，含104個(gè)氨基酸殘基。
Accession | |
GeneID | |
Source | Escherichia coli. |
Molecular Weight | Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids. |
Quantity | 10μg/50μg/1000μg |
AA Sequence | RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
參考文獻(xiàn)看起來(lái)：
1.Expression of apolipoprotein serum amyloid A mRNA in human atherosclerotic lesions and cultured vascular cells: implications for serum amyloid A function.作者：Meek, R. L.， Urieli-Shoval, S.， Benditt, E. P.
2. Serum amyloid A (SAA): An acute phase protein and apolipoprotein作者：Ernst Malle，Armin Steinmetz，John G. Raynes
3.[No significant influence of serum amyloid A1 genotypes on serum lipids or HDL clearance]作者：A Wada，T Yamada，K Itoh...
因?yàn)闀r(shí)間關(guān)系，就沒(méi)有給大家整理那么全面，大家可以用上面關(guān)鍵詞在國(guó)內(nèi)幾家數(shù)據(jù)庫(kù)中都可以檢索到文獻(xiàn)進(jìn)行參考。
突然想起來(lái)，這種微量的產(chǎn)品保存和使用上是有技巧的：
1. 收到產(chǎn)品后請(qǐng)立即按照說(shuō)明書(shū)推薦的條件保存。除非特別注明，相關(guān)產(chǎn)品均為凍干粉，由于微量的蛋白在凍干過(guò)程中沉積在管內(nèi)，形成很薄或不可見(jiàn)的蛋白層，所以在打開(kāi)管蓋前，我們建議在離心機(jī)中約8,000-12,000g離心10-30秒，使附著在管蓋或管壁上的蛋白聚集于管底。
2. 請(qǐng)根據(jù)實(shí)驗(yàn)?zāi)康牟凑债a(chǎn)品簡(jiǎn)介中Reconstitution一欄中的信息配制儲(chǔ)存液。大多數(shù)細(xì)胞因子或重組蛋白的凍干粉是非常容易溶解的，一般用移液槍的槍頭輕吹幾下或者輕輕搖晃瓶子，即可使細(xì)胞因子或重組蛋白完全溶解。請(qǐng)勿用vortex劇烈振蕩，以免蛋白變性而失活。
3. 具體的工作濃度請(qǐng)自行參考相關(guān)文獻(xiàn)，或者根據(jù)實(shí)驗(yàn)?zāi)康?，以及特定?xì)胞和動(dòng)物，通過(guò)實(shí)驗(yàn)進(jìn)行摸索和優(yōu)化
至此，相信大家對(duì)于科學(xué)家們，已經(jīng)是佩服的五體投地了，反正小編是佩服的不要不要的。好啦，這期就先到這里，下期再見(jiàn)啦
